Hotspot

rps15-trnN-GUU

SSC · 121791-128236 · 6446 bp

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Pi 0.0192

MarkerSeek score 54.10

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6446
variable_sites: 116
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	CGACCACAACAACCGAATTG	ACGGGGGATTCGATCTCATA	50.0	50.0	58.0	57.7	494 bp	0.5	57.3
rps15-trnN-GUU_p2	TATGAGCTGTCCCGCAAAAA	ACGGGGGATTCGATCTCATA	45.0	50.0	57.8	57.7	634 bp	0.5	56.0
rps15-trnN-GUU_p3	TTATGAGCTGTCCCGCAAAA	ACGGGGGATTCGATCTCATA	45.0	50.0	57.8	57.7	635 bp	0.5	56.0
rps15-trnN-GUU_p4	CGACCACAACAACCGAATTG	AACGGGGGATTCGATCTCAT	50.0	50.0	58.0	58.6	495 bp	0.5	55.1
rps15-trnN-GUU_p5	AACATTATGAGCTGTCCCGC	ACGGGGGATTCGATCTCATA	50.0	50.0	58.3	57.7	639 bp	0.5	54.9