Hotspot

psbN-psbH

LSC · 75383-75480 · 98 bp

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Pi 0.0213

MarkerSeek score 65.70

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 2
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GGCGTACCCGGTAAAACTTA	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.6	58.1	180-184 bp	1.0	78.7
psbN-psbH_p2	TAAGGCGTACCCGGTAAAAC	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.6	58.1	183-187 bp	1.0	78.7
psbN-psbH_p3	GGCGTACCCGGTAAAACTTA	CAGTGGTTTCAAAAAGGCCC	50.0	50.0	57.6	57.8	238-242 bp	1.0	77.1
psbN-psbH_p4	TAAGGCGTACCCGGTAAAAC	CAGTGGTTTCAAAAAGGCCC	50.0	50.0	57.6	57.8	241-245 bp	1.0	77.0
psbN-psbH_p5	GGCGTACCCGGTAAAACTTA	CCTACAGCAGTTCGTCTTGG	50.0	55.0	57.6	58.3	219-223 bp	1.0	76.9