Hotspot

petG-trnW-CCA

LSC · 67254-67402 · 149 bp

Back to Anthoshorea

Pi 0.0268

MarkerSeek score 67.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 4
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TTTACAATACAGGCGGGGTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	261 bp	1.0	80.2
petG-trnW-CCA_p2	TTTACAATACAGGCGGGGTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.9	58.0	239 bp	1.0	80.1
petG-trnW-CCA_p3	TTTACAATACAGGCGGGGTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.9	57.7	259 bp	1.0	77.5
petG-trnW-CCA_p4	TACAATACAGGCGGGGTGAT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.5	58.0	259 bp	1.0	77.1
petG-trnW-CCA_p5	TACAATACAGGCGGGGTGAT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.5	58.0	237 bp	1.0	77.1