Pi
0.0245
MarkerSeek score
66.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 867-1040 bp | 1.0 | 84.6 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 875-1048 bp | 1.0 | 84.0 |
| trnT-UGU-trnL-UAA_p3 | AAGAAATAGGGAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 923-1096 bp | 1.0 | 83.7 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 899-1072 bp | 1.0 | 83.7 |
| trnT-UGU-trnL-UAA_p5 | AAGAAATAGGGAAAGCCGGC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 931-1104 bp | 1.0 | 83.1 |