Hotspot

rps14-psaB

LSC · 37547-37675 · 129 bp

Back to Anthoshorea

Pi 0.0465

MarkerSeek score 67.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 6
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	AGTCAAGGAGGGAACTTTGC	TCTACCTCGGGCAAATTTGG	50.0	50.0	57.7	57.9	267 bp	1.0	78.6
rps14-psaB_p2	GTCAAGGAGGGAACTTTGCT	TCTACCTCGGGCAAATTTGG	50.0	50.0	57.7	57.9	266 bp	1.0	78.6
rps14-psaB_p3	AGTCAAGGAGGGAACTTTGC	CTACCTCGGGCAAATTTGGT	50.0	50.0	57.7	58.2	266 bp	1.0	78.3
rps14-psaB_p4	GTCAAGGAGGGAACTTTGCT	CTACCTCGGGCAAATTTGGT	50.0	50.0	57.7	58.2	265 bp	1.0	78.3
rps14-psaB_p5	GTCAAGGAGGGAACTTTGCT	CTTGATTGCCTCTACCTCGG	50.0	55.0	57.7	57.8	276 bp	1.0	77.7