Hotspot

psbZ-trnG-UCC

LSC · 36175-36783 · 609 bp

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Pi 0.0399

MarkerSeek score 82.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 23
pi: 0.0399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.9	57.8	763-853 bp	1.0	83.1
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	766-856 bp	1.0	82.4
psbZ-trnG-UCC_p3	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	762-852 bp	1.0	82.0
psbZ-trnG-UCC_p4	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	55.0	50.0	57.9	58.3	802-892 bp	1.0	81.7
psbZ-trnG-UCC_p5	TCCTGATGGTTGGGTGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	759-849 bp	1.0	81.4