Hotspot

ycf2

Genome · 33853-41034 · 7182 bp

Back to Anthoceros

Pi 0.0039

MarkerSeek score 43.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7182
variable_sites: 28
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCACGTTTGAATGGTGGAGG	ATATTGATTCGTGTTGCTCA	50.0	35.0	58.1	53.0	246 bp	1.0	41.3
ycf2_p2	TCACGTTTGAATGGTGGAGG	TATTGATTCGTGTTGCTCAT	50.0	35.0	58.1	53.0	245 bp	1.0	41.3
ycf2_p3	TCACGTTTGAATGGTGGAGG	AATATTGATTCGTGTTGCTCA	50.0	33.3	58.1	53.9	247 bp	1.0	41.3
ycf2_p4	TCACGTTTGAATGGTGGAGG	AAATATTGATTCGTGTTGCTCA	50.0	31.8	58.1	54.7	248 bp	1.0	41.3
ycf2_p5	TCACGTTTGAATGGTGGAGG	TCAGAGACTAAAAAGCAAGAAACA	50.0	33.3	58.1	56.8	343 bp	1.0	40.9