Hotspot

ccsA

Genome · 128098-129114 · 1017 bp

Back to Anthoceros

Pi 0.0059

MarkerSeek score 62.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 1017
variable_sites: 6
pi: 0.0059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAACCCATGACCTCCATCAC	ACCAGGGTTAAGTGGTTTCG	55.0	50.0	58.0	57.7	2056 bp	1.0	77.8
ccsA_p2	GAACCCATGACCTCCATCAC	CCAGGGTTAAGTGGTTTCGT	55.0	50.0	58.0	57.7	2055 bp	1.0	77.8
ccsA_p3	ACGCTGTTCTTAGGAAGCAG	CCAGGGTTAAGTGGTTTCGT	50.0	50.0	57.9	57.7	1841 bp	1.0	77.4
ccsA_p4	CGCTGTTCTTAGGAAGCAGT	ACCAGGGTTAAGTGGTTTCG	50.0	50.0	57.9	57.7	1841 bp	1.0	77.4
ccsA_p5	CGCTGTTCTTAGGAAGCAGT	CCAGGGTTAAGTGGTTTCGT	50.0	50.0	57.9	57.7	1840 bp	1.0	77.4