Hotspot

atpB

Genome · 70607-72085 · 1479 bp

Back to Anthoceros

Pi 0.00406

MarkerSeek score 61.10

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1479
variable_sites: 6
pi: 0.00406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GCATGATTGGGTAATACGCC	AGTAAGCGGTAACCCTTACT	50.0	45.0	57.0	55.6	1706 bp	1.0	53.0
atpB_p2	GCATGATTGGGTAATACGCC	AGCGGTAACCCTTACTAATCA	50.0	42.9	57.0	56.2	1702 bp	1.0	49.7
atpB_p3	GCATGATTGGGTAATACGCC	TGACTGATCAACCTGATACCA	50.0	42.9	57.0	56.1	1680 bp	1.0	49.0
atpB_p4	AGCATGATTGGGTAATACGCC	AGTAAGCGGTAACCCTTACT	47.6	45.0	58.5	55.6	1707 bp	1.0	48.9
atpB_p5	GCATGATTGGGTAATACGCC	ACCTGATACCAAAAATTGATGCG	50.0	39.1	57.0	58.0	1670 bp	1.0	48.4