Hotspot

accD

Genome · 75340-76281 · 942 bp

Back to Anthoceros

Pi 0.00531

MarkerSeek score 60.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 942
variable_sites: 5
pi: 0.00531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGGAATAACAGGGCTGAACT	CCAGTAGAGTGGTCAAAAGGA	45.0	47.6	56.1	57.0	1134 bp	1.0	48.9
accD_p2	AGGAATAACAGGGCTGAACT	TCCAGTAGAGTGGTCAAAAGG	45.0	47.6	56.1	57.0	1135 bp	1.0	48.9
accD_p3	AGGAATAACAGGGCTGAACTTC	CCAGTAGAGTGGTCAAAAGGA	45.5	47.6	58.0	57.0	1134 bp	1.0	47.6
accD_p4	AGGAATAACAGGGCTGAACTTC	TCCAGTAGAGTGGTCAAAAGG	45.5	47.6	58.0	57.0	1135 bp	1.0	47.6
accD_p5	AGGAATAACAGGGCTGAACTT	CCAGTAGAGTGGTCAAAAGGA	42.9	47.6	56.9	57.0	1134 bp	1.0	47.2