Hotspot

trnI-ndhB

Genome · 105239-107280 · 2042 bp

Back to Anthoceros

Pi 0.00539

MarkerSeek score 60.90

Primer available yes

Label trnI-ndhB

Feature Metadata

label_name: trnI-ndhB
feature_type: igs
region: Genome
length_bp: 2042
variable_sites: 11
pi: 0.00539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-ndhB_p1	TGAACCTGTGAATTCGCCAA	AACCCCGTCATTGCTATTGT	45.0	45.0	58.0	57.5	2160-2161 bp	1.0	76.3
trnI-ndhB_p2	TGAACCTGTGAATTCGCCAA	ACCCCGTCATTGCTATTGTT	45.0	45.0	58.0	57.5	2159-2160 bp	1.0	76.3
trnI-ndhB_p3	TTGAACCTGTGAATTCGCCA	AACCCCGTCATTGCTATTGT	45.0	45.0	58.0	57.5	2161-2162 bp	1.0	76.3
trnI-ndhB_p4	TTGAACCTGTGAATTCGCCA	ACCCCGTCATTGCTATTGTT	45.0	45.0	58.0	57.5	2160-2161 bp	1.0	76.3
trnI-ndhB_p5	TAACCGTTCAGCCATGGATG	ACCCCGTCATTGCTATTGTT	50.0	45.0	58.0	57.5	2122-2123 bp	1.0	76.2