Hotspot

trnP-psaJ

Genome · 84831-85344 · 514 bp

Back to Anthoceros

Pi 0.00784
MarkerSeek score 62.20
Primer available yes
Label trnP-psaJ

Feature Metadata

label_name
trnP-psaJ
feature_type
igs
region
Genome
length_bp
514
variable_sites
4
pi
0.00784
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-psaJ_p1 TACCAAACTGCGCTACATCC CCGAACCACAACGTAGCTAA 50.0 50.0 58.0 57.9 591-595 bp 1.0 80.4
trnP-psaJ_p2 GGGATGACAGGATTCGAACC CCGAACCACAACGTAGCTAA 55.0 50.0 58.0 57.9 641-645 bp 1.0 80.1
trnP-psaJ_p3 TACCAAACTGCGCTACATCC AACGTAGCTAACACAGGTGC 50.0 50.0 58.0 58.2 582-586 bp 1.0 79.3
trnP-psaJ_p4 AAACAAACGCGCTACCAAAC CCGAACCACAACGTAGCTAA 45.0 50.0 58.2 57.9 603-607 bp 1.0 79.1
trnP-psaJ_p5 CAAAACAAACGCGCTACCAA CCGAACCACAACGTAGCTAA 45.0 50.0 58.2 57.9 605-609 bp 1.0 79.1