Hotspot

trnF-ndhJ

Genome · 66469-66932 · 464 bp

Back to Anthoceros

Pi 0.00862

MarkerSeek score 60.90

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: Genome
length_bp: 464
variable_sites: 4
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	AATAGCCGGGATAGCTCAGT	TGGATTGGATGGCCTTTACG	50.0	50.0	58.0	57.9	610-614 bp	1.0	80.0
trnF-ndhJ_p2	ATAGCCGGGATAGCTCAGTT	TGGATTGGATGGCCTTTACG	50.0	50.0	58.0	57.9	609-613 bp	1.0	80.0
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATTGGATGGCCTTTACG	50.0	50.0	58.1	57.9	567-571 bp	1.0	79.7
trnF-ndhJ_p4	AATAGCCGGGATAGCTCAGT	GCCTGACAGTTGGATTGGAT	50.0	50.0	58.0	57.9	620-624 bp	1.0	79.3
trnF-ndhJ_p5	ATAGCCGGGATAGCTCAGTT	GCCTGACAGTTGGATTGGAT	50.0	50.0	58.0	57.9	619-623 bp	1.0	79.3