Hotspot

trnT-trnL

Genome · 64608-65343 · 736 bp

Back to Anthoceros

Pi 0.0215

MarkerSeek score 67.40

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: Genome
length_bp: 736
variable_sites: 15
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GACCTCTGAGCTAAACAGGC	ATTAAGTCCGCAGCGTCTAC	55.0	50.0	58.0	57.8	765-794 bp	1.0	83.4
trnT-trnL_p2	GACCTCTGAGCTAAACAGGC	TACCTATTCCGCCATACCCC	55.0	55.0	58.0	58.4	748-777 bp	1.0	82.3
trnT-trnL_p3	GACCTCTGAGCTAAACAGGC	CCTTCCCTCTTCATCCAACC	55.0	55.0	58.0	57.6	845-874 bp	1.0	81.1
trnT-trnL_p4	GACCATCGCATTACAAGTGC	ATTAAGTCCGCAGCGTCTAC	50.0	50.0	57.5	57.8	793-822 bp	1.0	79.6
trnT-trnL_p5	ATTACAAGTGCGGTGCTCTG	ATTAAGTCCGCAGCGTCTAC	50.0	50.0	58.6	57.8	784-813 bp	1.0	78.9