Hotspot

cysA-trnT

Genome · 44942-48845 · 3904 bp

Back to Anthoceros

Pi 0.00437

MarkerSeek score 50.60

Primer available yes

Label cysA-trnT

Feature Metadata

label_name: cysA-trnT
feature_type: igs
region: Genome
length_bp: 3904
variable_sites: 17
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysA-trnT_p1	AAAGAAAGTAGGTGCTGATT	AAGTCACCAAAGCCTTTTCT	35.0	40.0	52.4	55.4	245 bp	1.0	41.0
cysA-trnT_p2	AAAGAAAGTAGGTGCTGATT	AGTCACCAAAGCCTTTTCTT	35.0	40.0	52.4	55.4	244 bp	1.0	41.0
cysA-trnT_p3	AAAGAAAGTAGGTGCTGATT	AAAGTCACCAAAGCCTTTTCT	35.0	38.1	52.4	56.2	246 bp	1.0	41.0
cysA-trnT_p4	AAAGAAAGTAGGTGCTGATT	AAGTCACCAAAGCCTTTTCTT	35.0	38.1	52.4	56.2	245 bp	1.0	41.0
cysA-trnT_p5	AAAGAAAGTAGGTGCTGATT	AGTCACCAAAGCCTTTTCTTT	35.0	38.1	52.4	56.2	244 bp	1.0	41.0