Hotspot

ycf2-trnD

Genome · 41035-42855 · 1821 bp

Back to Anthoceros

Pi 0.0127

MarkerSeek score 53.60

Primer available yes

Label ycf2-trnD

Feature Metadata

label_name: ycf2-trnD
feature_type: igs
region: Genome
length_bp: 1821
variable_sites: 23
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnD_p1	TTACTTATGGGGCAAGACGC	CTCTCTTTCAAGGAGGCGAC	50.0	55.0	58.0	58.0	2820-2826 bp	1.0	80.6
ycf2-trnD_p2	TTACTTATGGGGCAAGACGC	CAACTGGTTAGAGTACCGCC	50.0	55.0	58.0	58.0	2371-2380 bp	1.0	80.6
ycf2-trnD_p3	TTACTTATGGGGCAAGACGC	ATCGTCTAGTGGCCTAGGAC	50.0	55.0	58.0	58.0	2842-2848 bp	1.0	80.5
ycf2-trnD_p4	TTACTTATGGGGCAAGACGC	CATCGTCTAGTGGCCTAGGA	50.0	55.0	58.0	58.0	2843-2849 bp	1.0	80.5
ycf2-trnD_p5	TTACTTATGGGGCAAGACGC	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.0	58.1	2364-2373 bp	1.0	80.1