primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	atpI	1	TCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACA	24	21	29.2	28.6	55.7	52.9	0.0	0.0	12.436344	15158	15904	828-828	1.000000	40.3
atpI_p2	atpI	2	TCACAATCAATTTTTCAAAGAGGT	TTGCGAAAAATGAAAACTTACA	24	22	29.2	27.3	55.7	53.7	0.0	0.0	12.568484	15158	15904	829-829	1.000000	40.3
atpI_p3	atpI	3	TTCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACA	25	21	28.0	28.6	56.4	52.9	0.0	0.0	12.769588	15158	15904	829-829	1.000000	40.3
atpI_p4	atpI	4	TCACAATCAATTTTTCAAAGAGGT	TTTGCGAAAAATGAAAACTTACA	24	23	29.2	26.1	55.7	54.5	0.0	0.0	12.774642	15158	15904	830-830	1.000000	40.3
atpI_p5	atpI	5	TCACAATCAATTTTTCAAAGAGGT	TGCGAAAAATGAAAACTTACAT	24	22	29.2	27.3	55.7	53.5	0.0	0.0	12.786679	15158	15904	828-828	1.000000	40.3
ORF51-trnK_p1	ORF51-trnK	1	GCTCAATGTCGGAGAGAAGG	GCACTTGAAAGCCGAGTACT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.269729	28025	28902	1270-1271	1.000000	78.8
ORF51-trnK_p2	ORF51-trnK	2	CAATGTCGGAGAGAAGGACG	GCACTTGAAAGCCGAGTACT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.288342	28025	28902	1267-1268	1.000000	78.7
ORF51-trnK_p3	ORF51-trnK	3	CTAGAGAAGGGGCTCAACCT	GCACTTGAAAGCCGAGTACT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.330203	28025	28902	1170-1171	1.000000	78.4
ORF51-trnK_p4	ORF51-trnK	4	AATGTCGGAGAGAAGGACGA	GCACTTGAAAGCCGAGTACT	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.367481	28025	28902	1266-1267	1.000000	78.1
ORF51-trnK_p5	ORF51-trnK	5	GGGGCTCAACCTACAGAAAC	GCACTTGAAAGCCGAGTACT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.380469	28025	28902	1162-1163	1.000000	78.0
trnH-ycf2_p1	trnH-ycf2	1	CATTATGCGCGGGTTCAATC	TCCTGTATGAAAATTTGTTTTCT	20	23	50.0	26.1	58.0	52.8	8.8	13.0	8.238439	33375	33852	547-551	1.000000	41.5
trnH-ycf2_p2	trnH-ycf2	2	TGGATTACGGCAGTGGATTG	TCCTGTATGAAAATTTGTTTTCT	20	23	50.0	26.1	58.0	52.8	0.0	13.0	8.238840	33375	33852	573-577	1.000000	41.4
trnH-ycf2_p3	trnH-ycf2	3	GGATTACGGCAGTGGATTGT	TCCTGTATGAAAATTTGTTTTCT	20	23	50.0	26.1	58.0	52.8	0.0	13.0	8.240269	33375	33852	572-576	1.000000	41.4
trnH-ycf2_p4	trnH-ycf2	4	GTGGATTACGGCAGTGGATT	TCCTGTATGAAAATTTGTTTTCT	20	23	50.0	26.1	58.0	52.8	0.0	13.0	8.240269	33375	33852	574-578	1.000000	41.4
trnH-ycf2_p5	trnH-ycf2	5	GATATTGAAAGGGCGGACGT	TCCTGTATGAAAATTTGTTTTCT	20	23	50.0	26.1	58.1	52.8	0.0	13.0	8.252417	33375	33852	600-604	1.000000	41.3
ycf2_p1	ycf2	1	TCACGTTTGAATGGTGGAGG	ATATTGATTCGTGTTGCTCA	20	20	50.0	35.0	58.1	53.0	0.0	0.0	5.106182	37967	38086	246-246	1.000000	41.3
ycf2_p2	ycf2	2	TCACGTTTGAATGGTGGAGG	TATTGATTCGTGTTGCTCAT	20	20	50.0	35.0	58.1	53.0	0.0	0.0	5.106182	37967	38086	245-245	1.000000	41.3
ycf2_p3	ycf2	3	TCACGTTTGAATGGTGGAGG	AATATTGATTCGTGTTGCTCA	20	21	50.0	33.3	58.1	53.9	0.0	0.0	5.198664	37967	38086	247-247	1.000000	41.3
ycf2_p4	ycf2	4	TCACGTTTGAATGGTGGAGG	AAATATTGATTCGTGTTGCTCA	20	22	50.0	31.8	58.1	54.7	0.0	0.0	5.372485	37967	38086	248-248	1.000000	41.3
ycf2_p5	ycf2	5	TCACGTTTGAATGGTGGAGG	TCAGAGACTAAAAAGCAAGAAACA	20	24	50.0	33.3	58.1	56.8	0.0	0.0	5.354665	37967	38086	343-343	1.000000	40.9
ycf2-trnD_p1	ycf2-trnD	1	TTACTTATGGGGCAAGACGC	CTCTCTTTCAAGGAGGCGAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035877	41035	42855	2820-2826	1.000000	80.6
ycf2-trnD_p2	ycf2-trnD	2	TTACTTATGGGGCAAGACGC	CAACTGGTTAGAGTACCGCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.038651	41035	42855	2371-2380	1.000000	80.6
ycf2-trnD_p3	ycf2-trnD	3	TTACTTATGGGGCAAGACGC	ATCGTCTAGTGGCCTAGGAC	20	20	50.0	55.0	58.0	58.0	0.0	14.8	0.048615	41035	42855	2842-2848	1.000000	80.5
ycf2-trnD_p4	ycf2-trnD	4	TTACTTATGGGGCAAGACGC	CATCGTCTAGTGGCCTAGGA	20	20	50.0	55.0	58.0	58.0	0.0	14.8	0.050020	41035	42855	2843-2849	1.000000	80.5
ycf2-trnD_p5	ycf2-trnD	5	TTACTTATGGGGCAAGACGC	TTAGAGTACCGCCCTGTCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105193	41035	42855	2364-2373	1.000000	80.1
cysA_p1	cysA	1	TGAAATTCTTTATGATGGGAGA	AGCAAGAATAGCAAAGAAAGCC	22	22	31.8	40.9	52.8	57.6	0.0	0.0	9.609015	43793	44941	1257-1257	1.000000	40.4
cysA_p2	cysA	2	TGAAATTCTTTATGATGGGAGA	GCAAGAATAGCAAAGAAAGCCT	22	22	31.8	40.9	52.8	57.6	0.0	0.0	9.609015	43793	44941	1256-1256	1.000000	40.4
cysA_p3	cysA	3	TGAAATTCTTTATGATGGGAGA	TCTCCAGAAAGCAAGAATAGCA	22	22	31.8	40.9	52.8	57.4	0.0	0.0	9.720247	43793	44941	1266-1266	1.000000	40.4
cysA_p4	cysA	4	TGAAATTCTTTATGATGGGAGAA	AGCAAGAATAGCAAAGAAAGCC	23	22	30.4	40.9	53.7	57.6	0.0	0.0	9.749870	43793	44941	1257-1257	1.000000	40.4
cysA_p5	cysA	5	TGAAATTCTTTATGATGGGAGAA	GCAAGAATAGCAAAGAAAGCCT	23	22	30.4	40.9	53.7	57.6	0.0	0.0	9.749870	43793	44941	1256-1256	1.000000	40.4
cysA-trnT_p1	cysA-trnT	1	AAAGAAAGTAGGTGCTGATT	AAGTCACCAAAGCCTTTTCT	20	20	35.0	40.0	52.4	55.4	0.0	0.0	8.278368	46375	46494	245-245	1.000000	41.0
cysA-trnT_p2	cysA-trnT	2	AAAGAAAGTAGGTGCTGATT	AGTCACCAAAGCCTTTTCTT	20	20	35.0	40.0	52.4	55.4	0.0	0.0	8.278368	46375	46494	244-244	1.000000	41.0
cysA-trnT_p3	cysA-trnT	3	AAAGAAAGTAGGTGCTGATT	AAAGTCACCAAAGCCTTTTCT	20	21	35.0	38.1	52.4	56.2	0.0	0.0	8.468342	46375	46494	246-246	1.000000	41.0
cysA-trnT_p4	cysA-trnT	4	AAAGAAAGTAGGTGCTGATT	AAGTCACCAAAGCCTTTTCTT	20	21	35.0	38.1	52.4	56.2	0.0	0.0	8.468342	46375	46494	245-245	1.000000	41.0
cysA-trnT_p5	cysA-trnT	5	AAAGAAAGTAGGTGCTGATT	AGTCACCAAAGCCTTTTCTTT	20	21	35.0	38.1	52.4	56.2	0.0	0.0	8.468342	46375	46494	244-244	1.000000	41.0
trnT-trnL_p1	trnT-trnL	1	GACCTCTGAGCTAAACAGGC	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.0	57.8	0.0	15.0	0.222445	64608	65343	765-794	1.000000	83.4
trnT-trnL_p2	trnT-trnL	2	GACCTCTGAGCTAAACAGGC	TACCTATTCCGCCATACCCC	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.369022	64608	65343	748-777	1.000000	82.3
trnT-trnL_p3	trnT-trnL	3	GACCTCTGAGCTAAACAGGC	CCTTCCCTCTTCATCCAACC	20	20	55.0	55.0	58.0	57.6	0.0	0.0	0.443980	64608	65343	845-874	1.000000	81.1
trnT-trnL_p4	trnT-trnL	4	GACCATCGCATTACAAGTGC	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.5	57.8	0.0	15.0	0.663898	64608	65343	793-822	1.000000	79.6
trnT-trnL_p5	trnT-trnL	5	ATTACAAGTGCGGTGCTCTG	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	58.6	57.8	0.0	15.0	0.762538	64608	65343	784-813	1.000000	78.9
trnF-ndhJ_p1	trnF-ndhJ	1	AATAGCCGGGATAGCTCAGT	TGGATTGGATGGCCTTTACG	20	20	50.0	50.0	58.0	57.9	2.2	3.3	0.068239	66469	66932	610-614	1.000000	80.0
trnF-ndhJ_p2	trnF-ndhJ	2	ATAGCCGGGATAGCTCAGTT	TGGATTGGATGGCCTTTACG	20	20	50.0	50.0	58.0	57.9	2.2	3.3	0.068239	66469	66932	609-613	1.000000	80.0
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGGATTGGATGGCCTTTACG	20	20	50.0	50.0	58.1	57.9	0.0	3.3	0.103230	66469	66932	567-571	1.000000	79.7
trnF-ndhJ_p4	trnF-ndhJ	4	AATAGCCGGGATAGCTCAGT	GCCTGACAGTTGGATTGGAT	20	20	50.0	50.0	58.0	57.9	2.2	0.9	0.151694	66469	66932	620-624	1.000000	79.3
trnF-ndhJ_p5	trnF-ndhJ	5	ATAGCCGGGATAGCTCAGTT	GCCTGACAGTTGGATTGGAT	20	20	50.0	50.0	58.0	57.9	2.2	0.9	0.151694	66469	66932	619-623	1.000000	79.3
atpB_p1	atpB	1	GCATGATTGGGTAATACGCC	AGTAAGCGGTAACCCTTACT	20	20	50.0	45.0	57.0	55.6	5.0	11.4	3.415681	70607	72085	1706-1706	1.000000	53.0
atpB_p2	atpB	2	GCATGATTGGGTAATACGCC	AGCGGTAACCCTTACTAATCA	20	21	50.0	42.9	57.0	56.2	5.0	0.0	3.826151	70607	72085	1702-1702	1.000000	49.7
atpB_p3	atpB	3	GCATGATTGGGTAATACGCC	TGACTGATCAACCTGATACCA	20	21	50.0	42.9	57.0	56.1	5.0	0.0	3.910382	70607	72085	1680-1680	1.000000	49.0
atpB_p4	atpB	4	AGCATGATTGGGTAATACGCC	AGTAAGCGGTAACCCTTACT	21	20	47.6	45.0	58.5	55.6	5.0	11.4	3.921891	70607	72085	1707-1707	1.000000	48.9
atpB_p5	atpB	5	GCATGATTGGGTAATACGCC	ACCTGATACCAAAAATTGATGCG	20	23	50.0	39.1	57.0	58.0	5.0	0.0	3.991857	70607	72085	1670-1670	1.000000	48.4
atpB-rbcL_p1	atpB-rbcL	1	TATGCCCCACATTCTTGGAG	TTGGTCTCGTAATCAGGGGT	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.787771	72086	73270	1298-1341	1.000000	76.8
atpB-rbcL_p2	atpB-rbcL	2	TATGCCCCACATTCTTGGAG	GTCTCCGTTTGTGGTGACAT	20	20	50.0	50.0	57.3	58.1	0.0	9.4	0.855802	72086	73270	1223-1266	1.000000	76.4
atpB-rbcL_p3	atpB-rbcL	3	ATGCCCCACATTCTTGGAGA	TTGGTCTCGTAATCAGGGGT	20	20	50.0	50.0	59.0	58.1	0.0	0.0	1.050946	72086	73270	1297-1340	1.000000	74.7
atpB-rbcL_p4	atpB-rbcL	4	TGCCCCACATTCTTGGAGAT	TTGGTCTCGTAATCAGGGGT	20	20	50.0	50.0	59.0	58.1	0.0	0.0	1.050946	72086	73270	1296-1339	1.000000	74.7
atpB-rbcL_p5	atpB-rbcL	5	GCCCCACATTCTTGGAGATA	TTGGTCTCGTAATCAGGGGT	20	20	50.0	50.0	57.0	58.1	0.0	0.0	1.082653	72086	73270	1295-1338	1.000000	74.4
accD_p1	accD	1	AGGAATAACAGGGCTGAACT	CCAGTAGAGTGGTCAAAAGGA	20	21	45.0	47.6	56.1	57.0	0.0	0.0	3.930302	75340	76281	1134-1134	1.000000	48.9
accD_p2	accD	2	AGGAATAACAGGGCTGAACT	TCCAGTAGAGTGGTCAAAAGG	20	21	45.0	47.6	56.1	57.0	0.0	3.3	3.930302	75340	76281	1135-1135	1.000000	48.9
accD_p3	accD	3	AGGAATAACAGGGCTGAACTTC	CCAGTAGAGTGGTCAAAAGGA	22	21	45.5	47.6	58.0	57.0	0.0	0.0	4.092874	75340	76281	1134-1134	1.000000	47.6
accD_p4	accD	4	AGGAATAACAGGGCTGAACTTC	TCCAGTAGAGTGGTCAAAAGG	22	21	45.5	47.6	58.0	57.0	0.0	3.3	4.092874	75340	76281	1135-1135	1.000000	47.6
accD_p5	accD	5	AGGAATAACAGGGCTGAACTT	CCAGTAGAGTGGTCAAAAGGA	21	21	42.9	47.6	56.9	57.0	0.0	0.0	4.148295	75340	76281	1134-1134	1.000000	47.2
petG-trnW_p1	petG-trnW	1	TGGTTGAAGCTTTGCTGTCT	TCGGTAGAACGTAGGTCTCC	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.086976	84383	84610	399-399	1.000000	80.3
petG-trnW_p2	petG-trnW	2	TGGTTGAAGCTTTGCTGTCT	CGGTAGAACGTAGGTCTCCA	20	20	45.0	55.0	57.9	58.3	0.0	0.0	0.314376	84383	84610	398-398	1.000000	78.5
petG-trnW_p3	petG-trnW	3	TGAAGCTTTGCTGTCTGGAA	TCGGTAGAACGTAGGTCTCC	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.376605	84383	84610	395-395	1.000000	78.0
petG-trnW_p4	petG-trnW	4	TTGAAGCTTTGCTGTCTGGA	TCGGTAGAACGTAGGTCTCC	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.376605	84383	84610	396-396	1.000000	78.0
petG-trnW_p5	petG-trnW	5	TGGTTGAAGCTTTGCTGTCT	CGCTCTTAGTTCAGTTCGGT	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.406434	84383	84610	414-414	1.000000	77.7
trnP-psaJ_p1	trnP-psaJ	1	TACCAAACTGCGCTACATCC	CCGAACCACAACGTAGCTAA	20	20	50.0	50.0	58.0	57.9	9.7	0.0	0.087379	84831	85344	591-595	1.000000	80.4
trnP-psaJ_p2	trnP-psaJ	2	GGGATGACAGGATTCGAACC	CCGAACCACAACGTAGCTAA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.113910	84831	85344	641-645	1.000000	80.1
trnP-psaJ_p3	trnP-psaJ	3	TACCAAACTGCGCTACATCC	AACGTAGCTAACACAGGTGC	20	20	50.0	50.0	58.0	58.2	9.7	0.0	0.226536	84831	85344	582-586	1.000000	79.3
trnP-psaJ_p4	trnP-psaJ	4	AAACAAACGCGCTACCAAAC	CCGAACCACAACGTAGCTAA	20	20	45.0	50.0	58.2	57.9	5.7	0.0	0.238751	84831	85344	603-607	1.000000	79.1
trnP-psaJ_p5	trnP-psaJ	5	CAAAACAAACGCGCTACCAA	CCGAACCACAACGTAGCTAA	20	20	45.0	50.0	58.2	57.9	5.7	0.0	0.240005	84831	85344	605-609	1.000000	79.1
trnI-ndhB_p1	trnI-ndhB	1	TGAACCTGTGAATTCGCCAA	AACCCCGTCATTGCTATTGT	20	20	45.0	45.0	58.0	57.5	16.1	0.0	0.533142	105239	107280	2160-2161	1.000000	76.3
trnI-ndhB_p2	trnI-ndhB	2	TGAACCTGTGAATTCGCCAA	ACCCCGTCATTGCTATTGTT	20	20	45.0	45.0	58.0	57.5	16.1	0.0	0.533142	105239	107280	2159-2160	1.000000	76.3
trnI-ndhB_p3	trnI-ndhB	3	TTGAACCTGTGAATTCGCCA	AACCCCGTCATTGCTATTGT	20	20	45.0	45.0	58.0	57.5	16.1	0.0	0.533142	105239	107280	2161-2162	1.000000	76.3
trnI-ndhB_p4	trnI-ndhB	4	TTGAACCTGTGAATTCGCCA	ACCCCGTCATTGCTATTGTT	20	20	45.0	45.0	58.0	57.5	16.1	0.0	0.533142	105239	107280	2160-2161	1.000000	76.3
trnI-ndhB_p5	trnI-ndhB	5	TAACCGTTCAGCCATGGATG	ACCCCGTCATTGCTATTGTT	20	20	50.0	45.0	58.0	57.5	24.3	0.0	0.544364	105239	107280	2122-2123	1.000000	76.2
ORF288_p1	ORF288	1	GGCTAATCCGAGATTTTGAGA	ACTATCATTTCCTTCTTTTCAGA	21	23	42.9	30.4	55.5	53.4	4.8	0.0	11.123982	126451	127317	1002-1003	1.000000	40.4
ORF288_p2	ORF288	2	GGCTAATCCGAGATTTTGAGA	AACTATCATTTCCTTCTTTTCAGA	21	24	42.9	29.2	55.5	54.2	4.8	0.0	11.326609	126451	127317	1003-1004	1.000000	40.4
ORF288_p3	ORF288	3	GGCTAATCCGAGATTTTGAGAA	ACTATCATTTCCTTCTTTTCAGA	22	23	40.9	30.4	56.2	53.4	4.8	0.0	11.367914	126451	127317	1002-1003	1.000000	40.4
ORF288_p4	ORF288	4	GGCTAATCCGAGATTTTGAGA	ACTATCATTTCCTTCTTTTCAGAT	21	24	42.9	29.2	55.5	54.0	4.8	0.0	11.531134	126451	127317	1002-1003	1.000000	40.4
ORF288_p5	ORF288	5	GGCTAATCCGAGATTTTGAGAA	AACTATCATTTCCTTCTTTTCAGA	22	24	40.9	29.2	56.2	54.2	4.8	0.0	11.570542	126451	127317	1003-1004	1.000000	40.4
ccsA_p1	ccsA	1	GAACCCATGACCTCCATCAC	ACCAGGGTTAAGTGGTTTCG	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.309711	128098	129114	2056-2056	1.000000	77.8
ccsA_p2	ccsA	2	GAACCCATGACCTCCATCAC	CCAGGGTTAAGTGGTTTCGT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.309711	128098	129114	2055-2055	1.000000	77.8
ccsA_p3	ccsA	3	ACGCTGTTCTTAGGAAGCAG	CCAGGGTTAAGTGGTTTCGT	20	20	50.0	50.0	57.9	57.7	10.7	0.0	0.351643	128098	129114	1841-1841	1.000000	77.4
ccsA_p4	ccsA	4	CGCTGTTCTTAGGAAGCAGT	ACCAGGGTTAAGTGGTTTCG	20	20	50.0	50.0	57.9	57.7	14.2	0.0	0.351643	128098	129114	1841-1841	1.000000	77.4
ccsA_p5	ccsA	5	CGCTGTTCTTAGGAAGCAGT	CCAGGGTTAAGTGGTTTCGT	20	20	50.0	50.0	57.9	57.7	14.2	0.0	0.351643	128098	129114	1840-1840	1.000000	77.4
chlL-trnN_p1	chlL-trnN	1	ACCAATTCCACCTTTCCCAT	AACAAAGCAAGCCCTTACCA	20	20	45.0	45.0	57.0	57.9	0.0	0.0	1.101150	144919	145781	1001-1046	1.000000	71.8
chlL-trnN_p2	chlL-trnN	2	ACCAATTCCACCTTTCCCAT	ACAAAGCAAGCCCTTACCAA	20	20	45.0	45.0	57.0	57.9	0.0	0.0	1.101150	144919	145781	1000-1045	1.000000	71.8
chlL-trnN_p3	chlL-trnN	3	ACCAATTCCACCTTTCCCAT	CCTCAGGTGGGATTTGAACC	20	20	45.0	55.0	57.0	58.2	0.0	0.0	1.181073	144919	145781	969-1014	1.000000	71.2
chlL-trnN_p4	chlL-trnN	4	CCACCTTTCCCATAAACTGCT	AACAAAGCAAGCCCTTACCA	21	20	47.6	45.0	58.2	57.9	0.0	0.0	1.272978	144919	145781	994-1039	1.000000	70.4
chlL-trnN_p5	chlL-trnN	5	CCACCTTTCCCATAAACTGCT	ACAAAGCAAGCCCTTACCAA	21	20	47.6	45.0	58.2	57.9	0.0	0.0	1.272978	144919	145781	993-1038	1.000000	70.4