Hotspot

rpl2-trnH-GUG

IRa · 155314-155355 · 42 bp

Back to Anthericum

Pi 0.0714

MarkerSeek score 82.00

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 42
variable_sites: 3
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	179 bp	1.0	80.1
rpl2-trnH-GUG_p2	TTATCCTGCGCTTGGAAGAC	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	178 bp	1.0	80.1
rpl2-trnH-GUG_p3	TTATCCTGCGCTTGGAAGAC	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	177 bp	1.0	80.1
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	193 bp	1.0	80.0
rpl2-trnH-GUG_p5	TTATCCTGCGCTTGGAAGAC	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	192 bp	1.0	80.0