Hotspot

rps15-ycf1

SSC · 124490-124941 · 452 bp

Back to Anthericum

Pi 0.0235
MarkerSeek score 73.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
452
variable_sites
10
pi
0.0235
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CTTGTCGAATAGCCGAGATGA TGATACCAATAACGGCAGTCA 47.6 42.9 57.6 57.1 542-559 bp 1.0 59.2
rps15-ycf1_p2 TCTTGTCGAATAGCCGAGATG TGATACCAATAACGGCAGTCA 47.6 42.9 57.6 57.1 543-560 bp 1.0 59.2
rps15-ycf1_p3 TGTCGAATAGCCGAGATGAA TGATACCAATAACGGCAGTCA 45.0 42.9 56.5 57.1 540-557 bp 1.0 57.8
rps15-ycf1_p4 TTGTCGAATAGCCGAGATGA TGATACCAATAACGGCAGTCA 45.0 42.9 56.5 57.1 541-558 bp 1.0 57.8
rps15-ycf1_p5 TTCTTGTCGAATAGCCGAGA TGATACCAATAACGGCAGTCA 45.0 42.9 56.4 57.1 544-561 bp 1.0 57.2