Hotspot

rpl32-trnL-UAG

SSC · 114484-115088 · 605 bp

Back to Anthericum

Pi 0.0314

MarkerSeek score 62.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 605
variable_sites: 19
pi: 0.0314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGTCAGTTTCTACCGGGGA	GAATTATGCAACCCCCGGAT	50.0	50.0	58.0	58.0	953 bp	0.5	59.8
rpl32-trnL-UAG_p2	AAGTCAGTTTCTACCGGGGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	706 bp	0.5	59.8
rpl32-trnL-UAG_p3	AAGTCAGTTTCTACCGGGGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	707 bp	0.5	59.8
rpl32-trnL-UAG_p4	AAGTCAGTTTCTACCGGGGA	TCGAACCGAGATGCTTTAGC	50.0	50.0	58.0	58.1	742 bp	0.5	59.2
rpl32-trnL-UAG_p5	AAGTCAGTTTCTACCGGGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	716 bp	0.5	58.8