Hotspot

trnH-GUG-rpl2

IRb · 84981-85022 · 42 bp

Back to Anthericum

Pi 0.0714

MarkerSeek score 82.00

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 42
variable_sites: 3
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	179 bp	1.0	80.1
trnH-GUG-rpl2_p2	TGGATCAAGGCAGTGGATTG	TTATCCTGCGCTTGGAAGAC	50.0	50.0	57.9	58.0	178 bp	1.0	80.1
trnH-GUG-rpl2_p3	GGATCAAGGCAGTGGATTGT	TTATCCTGCGCTTGGAAGAC	50.0	50.0	57.9	58.0	177 bp	1.0	80.1
trnH-GUG-rpl2_p4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	193 bp	1.0	80.0
trnH-GUG-rpl2_p5	CGGATGTAGCCAAGTGGATC	TTATCCTGCGCTTGGAAGAC	55.0	50.0	58.1	58.0	192 bp	1.0	80.0