Hotspot

rps3-rpl22

LSC · 84255-84307 · 53 bp

Back to Anthericum

Pi 0.0784

MarkerSeek score 78.40

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 53
variable_sites: 4
pi: 0.0784
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CCAAGTCTGAAACCAAGTGGA	TTAAGACCTAGGGCTCGAGG	47.6	55.0	58.1	57.9	202-206 bp	1.0	73.2
rps3-rpl22_p2	ACCAAGTCTGAAACCAAGTGG	TTAAGACCTAGGGCTCGAGG	47.6	55.0	58.3	57.9	203-207 bp	1.0	71.0
rps3-rpl22_p3	CCAAGTCTGAAACCAAGTGG	TTAAGACCTAGGGCTCGAGG	50.0	55.0	56.5	57.9	202-206 bp	1.0	70.2
rps3-rpl22_p4	TGGGTTGTACCAAGTCTGAA	TTAAGACCTAGGGCTCGAGG	45.0	55.0	56.3	57.9	211-215 bp	1.0	68.1
rps3-rpl22_p5	TTGGGTTGTACCAAGTCTGA	TTAAGACCTAGGGCTCGAGG	45.0	55.0	56.3	57.9	212-216 bp	1.0	68.1