Hotspot

psbH-petB

LSC · 75676-75789 · 114 bp

Back to Anthericum

Pi 0.0283

MarkerSeek score 70.50

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 3
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CTGGGGGACTACACCACTTA	ATCCGACGGGGTAGAATCAT	55.0	50.0	58.1	58.0	310-318 bp	1.0	82.3
psbH-petB_p2	GTCGCAATGGCTCTATTTGC	ATCCGACGGGGTAGAATCAT	50.0	50.0	57.9	58.0	285-293 bp	1.0	82.1
psbH-petB_p3	GGAGTCGCAATGGCTCTATT	ATCCGACGGGGTAGAATCAT	50.0	50.0	57.8	58.0	288-296 bp	1.0	81.0
psbH-petB_p4	ACTTATGGGAGTCGCAATGG	ATCCGACGGGGTAGAATCAT	50.0	50.0	57.7	58.0	295-303 bp	1.0	80.2
psbH-petB_p5	CCACTTATGGGAGTCGCAAT	ATCCGACGGGGTAGAATCAT	50.0	50.0	57.7	58.0	297-305 bp	1.0	80.2