Hotspot

clpP1-psbB

LSC · 72888-73334 · 447 bp

Back to Anthericum

Pi 0.0134

MarkerSeek score 48.70

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 6
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	GAACGGAGCCTGGATACTTG	AATGCGCCAAAACCAAAACA	55.0	40.0	58.1	58.0	1205-1215 bp	1.0	80.2
clpP1-psbB_p2	GAACGGAGCCTGGATACTTG	ATGCGCCAAAACCAAAACAA	55.0	40.0	58.1	58.0	1204-1214 bp	1.0	80.2
clpP1-psbB_p3	TGGCATGGCAGTTTGAGATT	AATGCGCCAAAACCAAAACA	45.0	40.0	58.1	58.0	1322-1357 bp	1.0	80.0
clpP1-psbB_p4	TGGCATGGCAGTTTGAGATT	ATGCGCCAAAACCAAAACAA	45.0	40.0	58.1	58.0	1321-1356 bp	1.0	80.0
clpP1-psbB_p5	TTGGCATGGCAGTTTGAGAT	ATGCGCCAAAACCAAAACAA	45.0	40.0	58.1	58.0	1322-1357 bp	1.0	80.0