Hotspot

psaJ-rpl33

LSC · 68032-68516 · 485 bp

Back to Anthericum

Pi 0.0147

MarkerSeek score 66.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 7
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGGTTTGGGTCTTTAGCGG	CCGGCGATTCCTTATTGACA	50.0	50.0	58.2	58.1	639-649 bp	1.0	80.4
psaJ-rpl33_p2	TTTGGGTCTTTAGCGGGTTT	CCGGCGATTCCTTATTGACA	45.0	50.0	57.6	58.1	635-645 bp	1.0	78.8
psaJ-rpl33_p3	GTTTGGGTCTTTAGCGGGTT	CCGGCGATTCCTTATTGACA	50.0	50.0	58.4	58.1	636-646 bp	1.0	78.6
psaJ-rpl33_p4	TCCCAGATGCCTTGTCATTC	CCGGCGATTCCTTATTGACA	50.0	50.0	57.6	58.1	592-602 bp	1.0	78.5
psaJ-rpl33_p5	CAGATGCCTTGTCATTCCCT	CCGGCGATTCCTTATTGACA	50.0	50.0	57.6	58.1	589-599 bp	1.0	78.4