Hotspot

rpoB-trnC-GCA

LSC · 26667-27609 · 943 bp

Back to Anthericum

Pi 0.018

MarkerSeek score 69.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 17
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGCCAAGCCCTGATTAATGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.3	58.5	1108-1122 bp	1.0	74.8
rpoB-trnC-GCA_p2	CGCCAAGCCCTGATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.3	57.5	1083-1097 bp	1.0	74.5
rpoB-trnC-GCA_p3	CGCCAAGCCCTGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.3	58.8	1120-1134 bp	1.0	72.5
rpoB-trnC-GCA_p4	CGCCAAGCCCTGATTAATGA	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.3	58.0	1084-1098 bp	1.0	70.4
rpoB-trnC-GCA_p5	CGCCAAGCCCTGATTAATGA	TTTTGTTGATCAGGCGACAC	50.0	45.0	58.3	56.9	1121-1135 bp	1.0	70.2