Hotspot

matK-trnK-UUU

LSC · 3237-3992 · 756 bp

Back to Anthericum

Pi 0.0137

MarkerSeek score 66.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 10
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGAGTAGCGGA	TCTGGGTTGCTAACTCAACG	50.0	50.0	57.8	57.8	849-875 bp	1.0	80.4
matK-trnK-UUU_p2	TAGGAGGTGTTGTTGCCAAG	TCTGGGTTGCTAACTCAACG	50.0	50.0	57.7	57.8	827-853 bp	1.0	80.1
matK-trnK-UUU_p3	GGAGGTGTTGTTGCCAAGAT	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.4	57.8	825-851 bp	1.0	79.2
matK-trnK-UUU_p4	ACTCCTGAAAGAGTAGCGGA	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.8	57.5	846-872 bp	1.0	78.0
matK-trnK-UUU_p5	ACTCCTGAAAGAGTAGCGGA	GCCGTAGTTGTACAGTGCAA	50.0	50.0	57.8	58.5	921-948 bp	1.0	77.5