Hotspot

ycf1

SSC · 121236-127004 · 5769 bp

Back to Anoectochilus

Pi 0.0105

MarkerSeek score 54.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5769
variable_sites: 166
pi: 0.0105
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACCAAAAGGAAGTTCCGCT	TCAACGGAGCTTATCGAGGA	45.0	50.0	57.9	58.2	1224-1257 bp	1.0	82.5
ycf1_p2	ACCAAAAGGAAGTTCCGCTT	TCAACGGAGCTTATCGAGGA	45.0	50.0	57.9	58.2	1223-1256 bp	1.0	82.5
ycf1_p3	TTCCTTCAAATCCTCGTGCC	TCAACGGAGCTTATCGAGGA	50.0	50.0	58.2	58.2	989-1022 bp	1.0	82.3
ycf1_p4	CTTCAAATCCTCGTGCCACT	TCAACGGAGCTTATCGAGGA	50.0	50.0	58.2	58.2	986-1019 bp	0.9	78.0
ycf1_p5	TTCAAATCCTCGTGCCACTC	TCAACGGAGCTTATCGAGGA	50.0	50.0	58.2	58.2	985-1018 bp	0.9	78.0