Hotspot

atpF

LSC · 10982-12341 · 1360 bp

Back to Anoectochilus

Pi 0.00772

MarkerSeek score 58.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1360
variable_sites: 32
pi: 0.00772
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCTCGAAGGGTTCCCATTAG	GCGGGTGGAGTGATACAAAA	55.0	50.0	58.0	58.2	1505-1515 bp	1.0	80.9
atpF_p2	AGCTCGAAGGGTTCCCATTA	GCGGGTGGAGTGATACAAAA	50.0	50.0	58.4	58.2	1506-1516 bp	1.0	77.9
atpF_p3	CTCGAAGGGTTCCCATTAGT	GCGGGTGGAGTGATACAAAA	50.0	50.0	56.6	58.2	1504-1514 bp	1.0	70.2
atpF_p4	GCTCGAAGGGTTCCCATTAG	GCGGGTGGAGTGATACAAA	55.0	52.6	58.0	57.5	1505-1515 bp	1.0	70.0
atpF_p5	AGCTCGAAGGGTTCCCATT	GCGGGTGGAGTGATACAAAA	52.6	50.0	58.6	58.2	1506-1516 bp	1.0	68.3