Hotspot

rpl32-trnL-UAG

SSC · 111323-111738 · 416 bp

Back to Anoectochilus

Pi 0.0178
MarkerSeek score 58.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
416
variable_sites
17
pi
0.0178
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.82
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAAAAGCTTTTACTGCCGC TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 401-1010 bp 1.0 76.6
rpl32-trnL-UAG_p2 GAAAAAGCTTTTACTGCCGCT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 400-1009 bp 1.0 76.6
rpl32-trnL-UAG_p3 AGAAAAAGCTTTTACTGCCGC AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 394-1003 bp 1.0 76.1
rpl32-trnL-UAG_p4 GAAAAAGCTTTTACTGCCGCT AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 393-1002 bp 1.0 76.1
rpl32-trnL-UAG_p5 GAAAAAGCTTTTACTGCCGCT CTAAGAGCAGCGTGTCTACC 42.9 55.0 58.0 57.8 398-1007 bp 1.0 76.0