Hotspot

petD-rpoA

LSC · 76174-76353 · 180 bp

Back to Anoectochilus

Pi 0.0174

MarkerSeek score 57.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 10
pi: 0.0174
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	ATTGGGCACTCTACAGAAGC	55.0	50.0	58.1	57.6	350-361 bp	1.0	79.5
petD-rpoA_p2	CGTCGTCCAGTAGCTACAAC	GGGCACTCTACAGAAGCATT	55.0	50.0	58.1	57.6	347-358 bp	1.0	79.5
petD-rpoA_p3	TTTTTGATCGGTACCGCAGT	ATTGGGCACTCTACAGAAGC	45.0	50.0	57.8	57.6	326-337 bp	1.0	79.0
petD-rpoA_p4	TTTTTGATCGGTACCGCAGT	GGGCACTCTACAGAAGCATT	45.0	50.0	57.8	57.6	323-334 bp	1.0	79.0
petD-rpoA_p5	TCGTCGTCCAGTAGCTACAA	GGGCACTCTACAGAAGCATT	50.0	50.0	58.2	57.6	348-359 bp	1.0	78.9