Hotspot

clpP-psbB

LSC · 70173-70657 · 485 bp

Back to Anoectochilus

Pi 0.00904

MarkerSeek score 69.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 14
pi: 0.00904
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	649-667 bp	1.0	84.2
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	644-662 bp	1.0	84.1
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	567-585 bp	1.0	83.9
clpP-psbB_p4	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	615-633 bp	1.0	83.8
clpP-psbB_p5	TATACGTCAACCCAAGCTGC	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	610-628 bp	1.0	83.7