Pi
0.0408
MarkerSeek score
82.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TCAATCATGGAAAGAGCCGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 696-1093 bp | 1.0 | 89.5 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 639-1036 bp | 1.0 | 89.2 |
| trnT-UGU-trnL-UAA_p3 | TCAATCATGGAAAGAGCCGG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 704-1101 bp | 1.0 | 89.0 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 647-1044 bp | 1.0 | 88.6 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 671-1068 bp | 1.0 | 88.1 |