Hotspot

psbM-trnD-GUC

LSC · 28763-29777 · 1015 bp

Back to Anoectochilus

Pi 0.0103

MarkerSeek score 67.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 29
pi: 0.0103
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTTAGAGCACC	50.0	47.6	57.9	58.0	1078-1129 bp	1.0	77.3
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTTAGAGCACC	45.0	47.6	58.3	58.0	1083-1134 bp	1.0	75.8
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTTAGAGCACC	45.0	47.6	58.3	58.0	1135-1186 bp	1.0	75.7
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTTAGAGCACC	45.0	47.6	57.6	58.0	1082-1133 bp	1.0	74.9
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTTAGAGCACC	45.0	47.6	58.5	58.0	1084-1135 bp	1.0	74.1