Hotspot

rpoB-trnC-GCA

LSC · 25874-27081 · 1208 bp

Back to Anoectochilus

Pi 0.00767

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 30
pi: 0.00767
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCCGATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	57.5	1328-1363 bp	1.0	78.5
rpoB-trnC-GCA_p2	CGTCAAGCCCCGATTAATGA	CGACACCCAGATTCGAACTG	50.0	55.0	58.1	58.6	1352-1387 bp	1.0	77.4
rpoB-trnC-GCA_p3	CGTCAAGCCCCGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.1	58.8	1365-1400 bp	1.0	76.5
rpoB-trnC-GCA_p4	CGTCAAGCCCCGATTAATGA	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.1	58.0	1329-1364 bp	1.0	74.4
rpoB-trnC-GCA_p5	CGTCAAGCCCCGATTAATGA	TTTTGTTGATCAGGCGACAC	50.0	45.0	58.1	56.9	1366-1401 bp	1.0	74.2