Hotspot

atpF-atpH

LSC · 12342-12769 · 428 bp

Back to Anoectochilus

Pi 0.017

MarkerSeek score 62.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 19
pi: 0.017
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCCCACGGAAACAAAAGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	59.3	58.0	519-533 bp	1.0	72.7
atpF-atpH_p2	GCCCCACGGAAACAAAAGAA	GGTCGTGGCATTAGCTCTTT	50.0	50.0	59.3	58.3	508-522 bp	1.0	71.0
atpF-atpH_p3	CCCCACGGAAACAAAAGAAT	ATTTATGGACTGGTCGTGGC	45.0	50.0	56.5	58.0	518-532 bp	1.0	70.8
atpF-atpH_p4	GCCCCACGGAAACAAAAGA	ATTTATGGACTGGTCGTGGC	52.6	50.0	58.6	58.0	519-533 bp	1.0	70.1
atpF-atpH_p5	GCCCCACGGAAACAAAAGAA	CAGAGGGCAAAATACGAGGT	50.0	50.0	59.3	57.6	578-592 bp	1.0	69.6