Hotspot

trnK-UUU-rps16

LSC · 4178-4870 · 693 bp

Back to Anoectochilus

Pi 0.0132

MarkerSeek score 60.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 28
pi: 0.0132
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1294-1335 bp	1.0	83.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1293-1334 bp	1.0	83.1
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	880-903 bp	1.0	82.9
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	879-902 bp	1.0	82.9
trnK-UUU-rps16_p5	AGGATCAGTCGCGGTCTTAT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.3	58.0	1379-1420 bp	1.0	81.8