Pi
0.254
MarkerSeek score
72.70
Primer available
yes
Label
ycf2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf2_p1 | ACGGGTGACTCATGATTTCG | ATACCGAACGATTTTCCGCA | 50.0 | 45.0 | 58.0 | 58.0 | 776-818 bp | 1.0 | 91.8 |
| ycf2_p2 | AACGTTCACGAGTGACCAAA | ATACCGAACGATTTTCCGCA | 45.0 | 45.0 | 58.0 | 58.0 | 1338-1380 bp | 1.0 | 91.8 |
| ycf2_p3 | ACGTTCACGAGTGACCAAAA | ATACCGAACGATTTTCCGCA | 45.0 | 45.0 | 58.0 | 58.0 | 1337-1379 bp | 1.0 | 91.8 |
| ycf2_p4 | CTCCTGAGCATTTGGGGATC | ATACCGAACGATTTTCCGCA | 55.0 | 45.0 | 58.0 | 58.0 | 819-861 bp | 1.0 | 91.7 |
| ycf2_p5 | GATCGGTGGGATCTGGTAGA | ATACCGAACGATTTTCCGCA | 55.0 | 45.0 | 58.0 | 58.0 | 1225-1234 bp | 0.5 | 71.9 |
| ycf2_p1 | ATACCGAACGATTTTCCGCA | ACGGGTGACTCATGATTTCG | 45.0 | 50.0 | 58.0 | 58.0 | 776-818 bp | 1.0 | 91.8 |
| ycf2_p2 | ATACCGAACGATTTTCCGCA | AACGTTCACGAGTGACCAAA | 45.0 | 45.0 | 58.0 | 58.0 | 1338-1380 bp | 1.0 | 91.8 |
| ycf2_p3 | ATACCGAACGATTTTCCGCA | ACGTTCACGAGTGACCAAAA | 45.0 | 45.0 | 58.0 | 58.0 | 1337-1379 bp | 1.0 | 91.8 |
| ycf2_p4 | ATACCGAACGATTTTCCGCA | CTCCTGAGCATTTGGGGATC | 45.0 | 55.0 | 58.0 | 58.0 | 819-861 bp | 1.0 | 91.7 |
| ycf2_p5 | ATACCGAACGATTTTCCGCA | GATCGGTGGGATCTGGTAGA | 45.0 | 55.0 | 58.0 | 58.0 | 1225-1234 bp | 0.5 | 71.9 |