Hotspot

rps18

LSC · 73132-73884 · 753 bp

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Pi 0.0603
MarkerSeek score 82.60
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
753
variable_sites
77
pi
0.0603
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 GGTTCATGGCCAAGGGTAAA CGTTTGTGGATCACTCGGAT 50.0 50.0 58.1 58.0 1453-1736 bp 1.0 88.5
rps18_p2 TAAGCGGATTTCGGGTTAGC CGTTTGTGGATCACTCGGAT 50.0 50.0 58.1 58.0 1552-1835 bp 1.0 88.3
rps18_p3 AACTTCAGCGAAACTCGTGT CGTTTGTGGATCACTCGGAT 45.0 50.0 58.1 58.0 1764-2047 bp 1.0 87.9
rps18_p4 ACTTCAGCGAAACTCGTGTT CGTTTGTGGATCACTCGGAT 45.0 50.0 58.1 58.0 1763-2046 bp 1.0 87.9
rps18_p5 TTCAGCGAAACTCGTGTTCA CGTTTGTGGATCACTCGGAT 45.0 50.0 58.1 58.0 1761-2044 bp 1.0 87.9