Pi
0.0852
MarkerSeek score
69.40
Primer available
yes
Label
rpoA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpoA_p1 | GTAGGCCTAGCGGTTCTAGA | ATGCTATTCATCTAGCCGCG | 55.0 | 50.0 | 58.0 | 58.0 | 1868-1955 bp | 1.0 | 91.8 |
| rpoA_p2 | GTAGGCCTAGCGGTTCTAGA | GGAGCGAATCTCAAGCAGAA | 55.0 | 50.0 | 58.0 | 58.0 | 1654-1741 bp | 1.0 | 91.7 |
| rpoA_p3 | GTAGGCCTAGCGGTTCTAGA | GGGCGTAGAATACCCAAAGG | 55.0 | 55.0 | 58.0 | 58.0 | 2037-2124 bp | 1.0 | 91.5 |
| rpoA_p4 | CTCGAGCCGGATGATGAAAA | ATGCTATTCATCTAGCCGCG | 50.0 | 50.0 | 58.1 | 58.0 | 2089-2176 bp | 1.0 | 91.5 |
| rpoA_p5 | ATCGGTACTGCAGTAGCTCT | ATGCTATTCATCTAGCCGCG | 50.0 | 50.0 | 57.9 | 58.0 | 1619-1706 bp | 1.0 | 91.5 |
| rpoA_p1 | ATGCTATTCATCTAGCCGCG | GTAGGCCTAGCGGTTCTAGA | 50.0 | 55.0 | 58.0 | 58.0 | 1868-1955 bp | 1.0 | 91.8 |
| rpoA_p2 | GGAGCGAATCTCAAGCAGAA | GTAGGCCTAGCGGTTCTAGA | 50.0 | 55.0 | 58.0 | 58.0 | 1654-1741 bp | 1.0 | 91.7 |
| rpoA_p3 | GGGCGTAGAATACCCAAAGG | GTAGGCCTAGCGGTTCTAGA | 55.0 | 55.0 | 58.0 | 58.0 | 2037-2124 bp | 1.0 | 91.5 |
| rpoA_p4 | ATGCTATTCATCTAGCCGCG | CTCGAGCCGGATGATGAAAA | 50.0 | 50.0 | 58.0 | 58.1 | 2089-2176 bp | 1.0 | 91.5 |
| rpoA_p5 | ATGCTATTCATCTAGCCGCG | ATCGGTACTGCAGTAGCTCT | 50.0 | 50.0 | 58.0 | 57.9 | 1619-1706 bp | 1.0 | 91.5 |