Hotspot

ycf2-trnL-CAA

IRa · 176571-177617 · 1047 bp

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Pi 0.296
MarkerSeek score 78.30
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRa
length_bp
1047
variable_sites
481
pi
0.296
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.81
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 TCGTTGATTTCTCGCCAGAG AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 1358-1359 bp 1.0 91.9
ycf2-trnL-CAA_p2 TCGTTGATTTCTCGCCAGAG TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 1360-1361 bp 1.0 91.9
ycf2-trnL-CAA_p3 CGTCGAGAACTCTTTGCAGA AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 1679-1710 bp 1.0 91.5
ycf2-trnL-CAA_p4 CGTCGAGAACTCTTTGCAGA TGAAATGGTAGACACGCGAG 50.0 50.0 57.9 58.0 1681-1712 bp 1.0 91.5
ycf2-trnL-CAA_p5 CTGCGCTCCTTTTCTTTTGG AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 1831-1862 bp 1.0 91.5