Hotspot

ycf1-trnN-GUU

IRa · 152963-153521 · 559 bp

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Pi 0.0888
MarkerSeek score 83.60
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
559
variable_sites
87
pi
0.0888
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 GGCTAACGCTGAACTTGGTA AAGCAAAAATGCGTTCCTCG 50.0 45.0 57.9 57.9 1425-2065 bp 1.0 85.9
ycf1-trnN-GUU_p2 GGCTAACGCTGAACTTGGTA CTTTTCCTCTGGGGGTAGGA 50.0 55.0 57.9 58.0 1670 bp 0.25 49.0
ycf1-trnN-GUU_p3 GGCTAACGCTGAACTTGGTA TCCTTTTCCTCTGGGGGTAG 50.0 55.0 57.9 58.0 1672 bp 0.25 49.0
ycf1-trnN-GUU_p4 GGAAGAGATAAGAGGGCCCT CTTTTCCTCTGGGGGTAGGA 55.0 55.0 57.9 58.0 1344 bp 0.25 48.8
ycf1-trnN-GUU_p5 GGAAGAGATAAGAGGGCCCT TCCTTTTCCTCTGGGGGTAG 55.0 55.0 57.9 58.0 1346 bp 0.25 48.8