Hotspot

ndhF-rpl32

SSC · 136185-136684 · 500 bp

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Pi 0.072
MarkerSeek score 68.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
500
variable_sites
62
pi
0.072
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGCAATTGGTCGTGTAAACC CATAAAGGGAGCGTCGTTCA 50.0 50.0 58.0 58.0 1456-1772 bp 1.0 85.8
ndhF-rpl32_p2 GGAAACTGTGCAGATTTCGC CATAAAGGGAGCGTCGTTCA 50.0 50.0 58.0 58.0 1677-1999 bp 1.0 85.5
ndhF-rpl32_p3 GGAAACTGTGCAGATTTCGC AAAGGGAGCGTCGTTCATAC 50.0 50.0 58.0 58.0 1674-1996 bp 1.0 85.5
ndhF-rpl32_p4 GGAAACTGTGCAGATTTCGC GGAGCGTCGTTCATACTTGT 50.0 50.0 58.0 58.0 1670-1992 bp 1.0 85.5
ndhF-rpl32_p5 CTGTGCAGATTTCGCAACTG CATAAAGGGAGCGTCGTTCA 50.0 50.0 58.0 58.0 1672-1994 bp 1.0 85.5