Hotspot

rpl36-infA

IRb · 85975-86119 · 145 bp

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Pi 0.0644
MarkerSeek score 68.30
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
IRb
length_bp
145
variable_sites
16
pi
0.0644
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 TTCTGCTTGAGATTCGCTCC AGAGGGGGCGTATAACCTAC 50.0 55.0 58.0 58.0 963-965 bp 0.5 66.1
rpl36-infA_p2 TTCTTCCTGCGGAGAATTCG TGATATCCGACCGAATGGGA 50.0 50.0 58.0 58.0 720 bp 0.25 49.9
rpl36-infA_p3 TTCTTCCTGCGGAGAATTCG AGAGGGGGCGTATAACCTAC 50.0 55.0 58.0 58.0 329 bp 0.25 49.9
rpl36-infA_p4 TTCTGCTTGAGATTCGCTCC TGATATCCGACCGAATGGGA 50.0 50.0 58.0 58.0 1354 bp 0.25 49.8
rpl36-infA_p5 TTCTTCCTGCGGAGAATTCG TGAATCGCTTCGAAACGCTA 50.0 45.0 58.0 58.0 481 bp 0.25 49.8