Hotspot

psbE-petL

LSC · 69890-70965 · 1076 bp

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Pi 0.047
MarkerSeek score 76.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1076
variable_sites
86
pi
0.047
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.90
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA TAGTTGAAGCAGCCAGGAGA 50.0 50.0 57.9 58.4 1118-1229 bp 1.0 86.7
psbE-petL_p2 CAACCCGCAATGAATAGGGA TTGAAGCAGCCAGGAGAAAA 50.0 45.0 57.9 57.6 1115-1226 bp 1.0 86.7
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGAAGCAGCCAGGAGAAAAC 50.0 50.0 57.9 58.4 1114-1225 bp 1.0 86.5
psbE-petL_p4 CAACCCGCAATGAATAGGGA GTTGAAGCAGCCAGGAGAAA 50.0 50.0 57.9 58.4 1116-1227 bp 1.0 86.4
psbE-petL_p5 CAACCCGCAATGAATAGGGA CAGCCAGGAGAAAACCGAAA 50.0 50.0 57.9 58.4 1109-1220 bp 1.0 86.4