primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accD_p1	accD	1	CCATTGCAATTGCCGGAAAT	GAAAACGGGGAGTGATCACA	20	20	45.0	50.0	58.0	57.8	26.9	27.0	0.205915	58478	60904	2239-2790	1.000000	90.4
accD_p2	accD	2	AAGAAGCCATTGCAATTGCC	GAAAACGGGGAGTGATCACA	20	20	45.0	50.0	57.9	57.8	26.8	27.0	0.282449	58478	60904	2245-2796	1.000000	89.7
accD_p3	accD	3	CCATTGCAATTGCCGGAAAT	ACGACTCCATCTACGACAGA	20	20	45.0	50.0	58.0	57.6	26.9	0.0	0.425255	58478	60904	2276-2827	1.000000	88.6
accD_p4	accD	4	CCATTGCAATTGCCGGAAAT	AAACGGGGAGTGATCACATC	20	20	45.0	50.0	58.0	57.6	26.9	27.0	0.430255	58478	60904	2237-2788	1.000000	88.6
accD_p5	accD	5	TATAGGGACCCGCATGCATA	GAAAACGGGGAGTGATCACA	20	20	50.0	50.0	58.1	57.8	19.6	27.0	0.233332	58478	60904	2853-2984	0.500000	67.6
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	TAGTTGAAGCAGCCAGGAGA	20	20	50.0	50.0	57.9	58.4	6.4	0.0	0.406825	69890	70965	1118-1229	1.000000	86.7
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	TTGAAGCAGCCAGGAGAAAA	20	20	50.0	45.0	57.9	57.6	6.4	0.0	0.414641	69890	70965	1115-1226	1.000000	86.7
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAAGCAGCCAGGAGAAAAC	20	20	50.0	50.0	57.9	58.4	6.4	0.0	0.442126	69890	70965	1114-1225	1.000000	86.5
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GTTGAAGCAGCCAGGAGAAA	20	20	50.0	50.0	57.9	58.4	6.4	0.0	0.442126	69890	70965	1116-1227	1.000000	86.4
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	CAGCCAGGAGAAAACCGAAA	20	20	50.0	50.0	57.9	58.4	6.4	0.0	0.446731	69890	70965	1109-1220	1.000000	86.4
rps18_p1	rps18	1	GGTTCATGGCCAAGGGTAAA	CGTTTGTGGATCACTCGGAT	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.078510	73132	73884	1453-1736	1.000000	88.5
rps18_p2	rps18	2	TAAGCGGATTTCGGGTTAGC	CGTTTGTGGATCACTCGGAT	20	20	50.0	50.0	58.1	58.0	5.9	0.0	0.066113	73132	73884	1552-1835	1.000000	88.3
rps18_p3	rps18	3	AACTTCAGCGAAACTCGTGT	CGTTTGTGGATCACTCGGAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.074213	73132	73884	1764-2047	1.000000	87.9
rps18_p4	rps18	4	ACTTCAGCGAAACTCGTGTT	CGTTTGTGGATCACTCGGAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.074213	73132	73884	1763-2046	1.000000	87.9
rps18_p5	rps18	5	TTCAGCGAAACTCGTGTTCA	CGTTTGTGGATCACTCGGAT	20	20	45.0	50.0	58.1	58.0	2.9	0.0	0.080915	73132	73884	1761-2044	1.000000	87.9
rpoA_p1	rpoA	1	GTAGGCCTAGCGGTTCTAGA	ATGCTATTCATCTAGCCGCG	20	20	55.0	50.0	58.0	58.0	14.4	0.0	0.023357	84062	85078	1868-1955	1.000000	91.8
rpoA_p2	rpoA	2	GTAGGCCTAGCGGTTCTAGA	GGAGCGAATCTCAAGCAGAA	20	20	55.0	50.0	58.0	58.0	14.4	0.0	0.037281	84062	85078	1654-1741	1.000000	91.7
rpoA_p3	rpoA	3	GTAGGCCTAGCGGTTCTAGA	GGGCGTAGAATACCCAAAGG	20	20	55.0	55.0	58.0	58.0	14.4	0.0	0.058373	84062	85078	2037-2124	1.000000	91.5
rpoA_p4	rpoA	4	CTCGAGCCGGATGATGAAAA	ATGCTATTCATCTAGCCGCG	20	20	50.0	50.0	58.1	58.0	5.0	0.0	0.061838	84062	85078	2089-2176	1.000000	91.5
rpoA_p5	rpoA	5	ATCGGTACTGCAGTAGCTCT	ATGCTATTCATCTAGCCGCG	20	20	50.0	50.0	57.9	58.0	38.7	0.0	0.061862	84062	85078	1619-1706	1.000000	91.5
rpl36-infA_p1	rpl36-infA	1	TTCTGCTTGAGATTCGCTCC	AGAGGGGGCGTATAACCTAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.022541	85975	86119	963-965	0.500000	66.1
rpl36-infA_p2	rpl36-infA	2	TTCTTCCTGCGGAGAATTCG	TGATATCCGACCGAATGGGA	20	20	50.0	50.0	58.0	58.0	5.1	0.0	0.014082	85975	86119	720-720	0.250000	49.9
rpl36-infA_p3	rpl36-infA	3	TTCTTCCTGCGGAGAATTCG	AGAGGGGGCGTATAACCTAC	20	20	50.0	55.0	58.0	58.0	5.1	0.0	0.016950	85975	86119	329-329	0.250000	49.9
rpl36-infA_p4	rpl36-infA	4	TTCTGCTTGAGATTCGCTCC	TGATATCCGACCGAATGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019673	85975	86119	1354-1354	0.250000	49.8
rpl36-infA_p5	rpl36-infA	5	TTCTTCCTGCGGAGAATTCG	TGAATCGCTTCGAAACGCTA	20	20	50.0	45.0	58.0	58.0	5.1	15.8	0.024811	85975	86119	481-481	0.250000	49.8
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	TCGTTGATTTCTCGCCAGAG	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.011539	93051	94097	1358-1359	1.000000	91.9
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	TCGTTGATTTCTCGCCAGAG	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.012909	93051	94097	1360-1361	1.000000	91.9
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AAATGGTAGACACGCGAGAC	CGTCGAGAACTCTTTGCAGA	20	20	50.0	50.0	58.0	57.9	15.9	0.0	0.059709	93051	94097	1679-1710	1.000000	91.5
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	CGTCGAGAACTCTTTGCAGA	20	20	50.0	50.0	58.0	57.9	15.9	0.0	0.061080	93051	94097	1681-1712	1.000000	91.5
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	CTGCGCTCCTTTTCTTTTGG	20	20	50.0	50.0	58.0	57.9	15.9	9.7	0.063925	93051	94097	1831-1862	1.000000	91.5
ycf2_p1	ycf2	1	ACGGGTGACTCATGATTTCG	ATACCGAACGATTTTCCGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023082	97615	97734	776-818	1.000000	91.8
ycf2_p2	ycf2	2	AACGTTCACGAGTGACCAAA	ATACCGAACGATTTTCCGCA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.025874	97615	97734	1338-1380	1.000000	91.8
ycf2_p3	ycf2	3	ACGTTCACGAGTGACCAAAA	ATACCGAACGATTTTCCGCA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.025874	97615	97734	1337-1379	1.000000	91.8
ycf2_p4	ycf2	4	CTCCTGAGCATTTGGGGATC	ATACCGAACGATTTTCCGCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.034482	97615	97734	819-861	1.000000	91.7
ycf2_p5	ycf2	5	GATCGGTGGGATCTGGTAGA	ATACCGAACGATTTTCCGCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016138	97615	97734	1225-1234	0.500000	71.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AAGCAAAAATGCGTTCCTCG	GGCTAACGCTGAACTTGGTA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.139427	117147	117705	1425-2065	1.000000	86.4
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	CTTTTCCTCTGGGGGTAGGA	GGCTAACGCTGAACTTGGTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.122104	117147	117705	1670-1670	0.250000	49.0
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	TCCTTTTCCTCTGGGGGTAG	GGCTAACGCTGAACTTGGTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.122104	117147	117705	1672-1672	0.250000	49.0
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CTTTTCCTCTGGGGGTAGGA	AGGAAGAGATAAGAGGGCCC	20	20	55.0	55.0	58.0	57.9	0.0	16.5	0.143908	117147	117705	1345-1345	0.250000	48.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CTTTTCCTCTGGGGGTAGGA	GGAAGAGATAAGAGGGCCCT	20	20	55.0	55.0	58.0	57.9	0.0	29.2	0.143908	117147	117705	1344-1344	0.250000	48.8
ndhF-rpl32_p1	ndhF-rpl32	1	CGCAATTGGTCGTGTAAACC	CATAAAGGGAGCGTCGTTCA	20	20	50.0	50.0	58.0	58.0	7.7	11.3	0.046166	136185	136684	1456-1772	1.000000	85.8
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAACTGTGCAGATTTCGC	CATAAAGGGAGCGTCGTTCA	20	20	50.0	50.0	58.0	58.0	6.2	11.3	0.035479	136185	136684	1677-1999	1.000000	85.5
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAACTGTGCAGATTTCGC	AAAGGGAGCGTCGTTCATAC	20	20	50.0	50.0	58.0	58.0	6.2	11.3	0.036867	136185	136684	1674-1996	1.000000	85.5
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAACTGTGCAGATTTCGC	GGAGCGTCGTTCATACTTGT	20	20	50.0	50.0	58.0	58.0	6.2	11.3	0.038727	136185	136684	1670-1992	1.000000	85.5
ndhF-rpl32_p5	ndhF-rpl32	5	CTGTGCAGATTTCGCAACTG	CATAAAGGGAGCGTCGTTCA	20	20	50.0	50.0	58.0	58.0	0.0	11.3	0.044836	136185	136684	1672-1994	1.000000	85.5
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	GGCTAACGCTGAACTTGGTA	AAGCAAAAATGCGTTCCTCG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.139427	152963	153521	1425-2065	1.000000	85.9
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GGCTAACGCTGAACTTGGTA	CTTTTCCTCTGGGGGTAGGA	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.122104	152963	153521	1670-1670	0.250000	49.0
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	GGCTAACGCTGAACTTGGTA	TCCTTTTCCTCTGGGGGTAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.122104	152963	153521	1672-1672	0.250000	49.0
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GGAAGAGATAAGAGGGCCCT	CTTTTCCTCTGGGGGTAGGA	20	20	55.0	55.0	57.9	58.0	29.2	0.0	0.143908	152963	153521	1344-1344	0.250000	48.8
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GGAAGAGATAAGAGGGCCCT	TCCTTTTCCTCTGGGGGTAG	20	20	55.0	55.0	57.9	58.0	29.2	0.0	0.143908	152963	153521	1346-1346	0.250000	48.8
ycf2_p1	ycf2	1	ATACCGAACGATTTTCCGCA	ACGGGTGACTCATGATTTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.023082	172853	172972	776-818	1.000000	91.8
ycf2_p2	ycf2	2	ATACCGAACGATTTTCCGCA	AACGTTCACGAGTGACCAAA	20	20	45.0	45.0	58.0	58.0	0.0	6.3	0.025874	172853	172972	1338-1380	1.000000	91.8
ycf2_p3	ycf2	3	ATACCGAACGATTTTCCGCA	ACGTTCACGAGTGACCAAAA	20	20	45.0	45.0	58.0	58.0	0.0	6.3	0.025874	172853	172972	1337-1379	1.000000	91.8
ycf2_p4	ycf2	4	ATACCGAACGATTTTCCGCA	CTCCTGAGCATTTGGGGATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.034482	172853	172972	819-861	1.000000	91.7
ycf2_p5	ycf2	5	ATACCGAACGATTTTCCGCA	GATCGGTGGGATCTGGTAGA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.016138	172853	172972	1225-1234	0.500000	71.9
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	TCGTTGATTTCTCGCCAGAG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.011539	176571	177617	1358-1359	1.000000	91.9
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TCGTTGATTTCTCGCCAGAG	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.012909	176571	177617	1360-1361	1.000000	91.9
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	CGTCGAGAACTCTTTGCAGA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.9	58.0	0.0	15.9	0.059709	176571	177617	1679-1710	1.000000	91.5
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	CGTCGAGAACTCTTTGCAGA	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	15.9	0.061080	176571	177617	1681-1712	1.000000	91.5
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	CTGCGCTCCTTTTCTTTTGG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.9	58.0	9.7	15.9	0.063925	176571	177617	1831-1862	1.000000	91.5
rpoA_p1	rpoA	1	ATGCTATTCATCTAGCCGCG	GTAGGCCTAGCGGTTCTAGA	20	20	50.0	55.0	58.0	58.0	0.0	14.4	0.023357	185590	186606	1868-1955	1.000000	91.8
rpoA_p2	rpoA	2	GGAGCGAATCTCAAGCAGAA	GTAGGCCTAGCGGTTCTAGA	20	20	50.0	55.0	58.0	58.0	0.0	14.4	0.037281	185590	186606	1654-1741	1.000000	91.7
rpoA_p3	rpoA	3	GGGCGTAGAATACCCAAAGG	GTAGGCCTAGCGGTTCTAGA	20	20	55.0	55.0	58.0	58.0	0.0	14.4	0.058373	185590	186606	2037-2124	1.000000	91.5
rpoA_p4	rpoA	4	ATGCTATTCATCTAGCCGCG	CTCGAGCCGGATGATGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	5.0	0.061838	185590	186606	2089-2176	1.000000	91.5
rpoA_p5	rpoA	5	ATGCTATTCATCTAGCCGCG	ATCGGTACTGCAGTAGCTCT	20	20	50.0	50.0	58.0	57.9	0.0	38.7	0.061862	185590	186606	1619-1706	1.000000	91.5