Hotspot

cemA

LSC · 62473-63165 · 693 bp

Pi 0.00265

MarkerSeek score 39.00

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 693
variable_sites: 3
pi: 0.00265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GTCGACGAATGAAGTGGGTT	AGATTAATATCGGTGGATCATT	50.0	31.8	58.2	52.5	760 bp	1.0	40.3
cemA_p2	GTCGACGAATGAAGTGGGTT	TGATTAGATTAATATCGGTGGATCA	50.0	32.0	58.2	55.5	765 bp	1.0	40.3
cemA_p3	GTCGACGAATGAAGTGGGTT	AGATTAATATCGGTGGATCATTT	50.0	30.4	58.2	53.4	760 bp	1.0	40.3
cemA_p4	GAGTCGACGAATGAAGTGGG	AGATTAATATCGGTGGATCATT	55.0	31.8	58.4	52.5	762 bp	1.0	40.3
cemA_p5	GAGTCGACGAATGAAGTGGG	TGATTAGATTAATATCGGTGGATCA	55.0	32.0	58.4	55.5	767 bp	1.0	40.3