Hotspot

rps15-ycf1

SSC · 123513-123903 · 391 bp

Back to Anna

Pi 0.00426
MarkerSeek score 33.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
391
variable_sites
3
pi
0.00426
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCAACGCGTTACATAAGG ACAGCAACCAAGTTTCGTCT 50.0 45.0 58.0 58.0 1171-1172 bp 1.0 80.3
rps15-ycf1_p2 TCCCAACGCGTTACATAAGG GAAGCCAGTTCACCACATCA 50.0 50.0 58.0 58.1 1358-1359 bp 1.0 80.1
rps15-ycf1_p3 TCCTAGTTGTTCCGGTCCAT ACAGCAACCAAGTTTCGTCT 50.0 45.0 58.1 58.0 1229-1230 bp 1.0 80.0
rps15-ycf1_p4 TCGCCGTCGAGAGTAACTAT ACAGCAACCAAGTTTCGTCT 50.0 45.0 58.1 58.0 1063-1064 bp 1.0 80.0
rps15-ycf1_p5 AACACCATGCATTGATGGGT ACAGCAACCAAGTTTCGTCT 45.0 45.0 58.1 58.0 1034-1035 bp 1.0 80.0