Hotspot

ndhG-ndhI

SSC · 118896-119237 · 342 bp

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Pi 0.00682
MarkerSeek score 57.90
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
342
variable_sites
4
pi
0.00682
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCCCAATGAAAAGGCCGAAT CGGTTACCGGTGTCAATAATTG 45.0 45.5 57.8 57.7 540 bp 1.0 60.6
ndhG-ndhI_p2 TCCCAATGAAAAGGCCGAAT CGGTTACCGGTGTCAATAAT 45.0 45.0 57.8 55.6 540 bp 1.0 59.2
ndhG-ndhI_p3 AGACCCGATCCCAAAAAGAC CGGTTACCGGTGTCAATAATTG 50.0 45.5 57.5 57.7 476 bp 1.0 58.6
ndhG-ndhI_p4 GACCCGATCCCAAAAAGACT CGGTTACCGGTGTCAATAATTG 50.0 45.5 57.5 57.7 475 bp 1.0 58.6
ndhG-ndhI_p5 TCCCAATGAAAAGGCCGAAT CGGTTACCGGTGTCAATAATT 45.0 42.9 57.8 56.3 540 bp 1.0 57.5