Hotspot

ndhD-psaC

SSC · 117271-117366 · 96 bp

Pi 0.0434

MarkerSeek score 82.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 8
pi: 0.0434
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGAACCTGCAGATATTGGCA	CCGACCGATTTCTTGAGTGT	45.0	50.0	57.2	57.9	243 bp	1.0	75.6
ndhD-psaC_p2	TGAACCTGCAGATATTGGCA	GACCGAAGACTGTGTTGGTT	45.0	50.0	57.2	58.1	286 bp	1.0	75.4
ndhD-psaC_p3	TGAACCTGCAGATATTGGCA	AAGGACCGAAGACTGTGTTG	45.0	50.0	57.2	57.8	289 bp	1.0	73.9
ndhD-psaC_p4	TGAACCTGCAGATATTGGCA	CAAGGACCGAAGACTGTGTT	45.0	50.0	57.2	57.8	290 bp	1.0	73.8
ndhD-psaC_p5	TGAACCTGCAGATATTGGCA	ACTGTGTTGGTTGTAAGCGA	45.0	45.0	57.2	57.7	278 bp	1.0	73.3